All-In-One Master Mix Substrate Bag 2kg Commercial Grade

Grow premium wood-loving mushrooms with confidence using the Commercial Grade 2kg All-In-One Master Mix Substrate Bag. Professionally prepared for growers who want reliable colonisation, strong mycelial growth, and outstanding harvest potential, this all-in-one growing bag combines premium hydrated rye grain with a nutrient-rich 50/50 Master Mix of hardwood sawdust and soy hulls.

Designed for use with your own liquid culture or spore syringe, the layered construction allows both the spawn run and fruiting substrate to be contained within a single sterile bag, simplifying the cultivation process while reducing handling. Each bag is commercially sterilised using monitored core temperatures and sealed in a Class 100 cleanroom environment to deliver exceptional quality and minimise contamination risks.

Features

• Commercial-grade 2kg all-in-one mushroom growing substrate bag.
• Premium hydrated rye grain starter layer for rapid colonisation.
• 50/50 Master Mix consisting of hardwood sawdust and soy hulls.
• Suitable for wood-loving mushroom species including Oyster, Lion's Mane and Chestnut mushrooms.
• Ready for inoculation using liquid culture or spore syringe.
• Built-in self-healing injection port.
• High-quality filter patch for gas exchange.
• Professionally sterilised using monitored core-temperature autoclave processing.
• Sealed in a Class 100 cleanroom laboratory.
• Designed for both spawn run and bulk fruiting in one bag.
• Reduces handling by eliminating the need for separate grain spawn and bulk substrate.
• Ideal for hobby growers and commercial-scale cultivation.

Why You Should Buy

Whether you are new to mushroom cultivation or an experienced grower looking for consistent results, this All-In-One Master Mix bag provides a simple and efficient solution. The premium rye grain promotes fast colonisation, while the nutrient-rich Master Mix supports vigorous mycelial growth and impressive yields. Manufactured using the same commercial sterilisation standards used on a professional mushroom farm, every bag is prepared to maximise reliability and minimise contamination, allowing you to focus on growing healthy, productive mushroom harvests.

How to Use

1. Wipe the self-healing injection port with an isopropyl alcohol swab.
2. Flame sterilise your liquid culture or spore syringe needle until red hot and allow it to cool for several seconds.
3. Inject 4–10ml of culture into the rye grain layer through the injection port.
4. Store upright in a clean, dark location between 16–20°C with good airflow.
5. Allow the rye grain layer to become fully colonised before breaking up the grain and mixing it thoroughly into the Master Mix layer.
6. Continue incubating until the entire substrate forms a solid, fully colonised block.
7. Cut a small "X" or slit into the side of the bag and introduce fresh air and humidity to begin fruiting.

Storage & Care

• Always store bags standing upright.
• Keep the filter patch dry at all times.
• Ensure adequate airflow around the bag.
• Store below 20°C in a cool, dark location.
• Do not refrigerate before inoculation.
• Avoid temperatures above 22–24°C to reduce condensation.
• Maintain a clean working environment and wear freshly cleaned clothing during handling.

Arrival & Warranty Information

• Unbox immediately upon arrival.
• Allow the unopened bag to rest for one week before inoculation.
• Inspect for signs of contamination or shipping damage during this period.
• If contamination develops before inoculation, contact us with photos within two weeks of delivery for warranty assessment.
• Bags inoculated before the recommended resting period are not covered under warranty.
• Contamination occurring after inoculation is generally the result of the inoculation process, handling, storage conditions or contaminated cultures and is not covered by warranty.

Specifications

• Product Type: All-In-One Mushroom Growing Substrate Bag
• Weight: 2kg
• Grain Layer: Premium Hydrated Rye Grain
• Bulk Substrate: 50% Hardwood Sawdust / 50% Soy Hulls (Master Mix)
• Sterilisation Method: Commercial Autoclave with Core Temperature Monitoring
• Sealing Environment: Class 100 Cleanroom
• Injection Port: Self-Healing
• Filter Patch: Integrated Gas Exchange Filter
• Suitable For: Liquid Culture (LC) and Spore Syringe Inoculation
• Compatible Mushroom Types: Oyster, Lion's Mane, Chestnut and other wood-loving mushroom species

Package Includes

• 1 × Commercial Grade 2kg All-In-One Master Mix Substrate Bag

FAQs

What mushroom species is this bag suitable for?
This substrate is designed for wood-loving mushroom species including Oyster, Lion's Mane and Chestnut mushrooms.

Is the bag ready to use?
Yes. The bag is fully sterilised and ready to be inoculated with your own liquid culture or spore syringe.

Does the bag include mushroom culture or spores?
No. Liquid culture and spore syringes are sold separately.

Why should I let the bag rest after delivery?
Allowing the bag to rest for one week helps identify any damage or contamination that may have occurred during shipping before you use your culture.

Why must the filter patch stay dry?
The filter functions correctly only when dry. Excess moisture can increase the risk of contamination.

What temperature should I store the bag at?
For best results, keep the bag in a dark area between 16–20°C with good airflow and avoid temperatures above 22–24°C.

When should I mix the grain and substrate?
Only after the rye grain layer has become completely colonised with healthy white mycelium.

Is this bag suitable for beginners?
Yes. The all-in-one design simplifies the growing process by combining the grain spawn and fruiting substrate into a single bag.